Exons are shown as black boxes, the transcription initiation site as an arrow, and sgRNA target sites as green triangles. (D) A schematic representation of the séan gene showing the sgRNA target sites. (C) Séan expression profile based on four time points during the 元 stage, data retrieved from our previous study (Ou et al. (B) RNA in situ hybridization of Drosophila embryo with a séan-probe. Four other ZAD-zinc finger protein genes are shown in cyan. Boxed arrows represent gene spans and their directions. ![]() Numbers indicate the nucleotide positions at the 85A9 cytological location of the chromosome 3R scaffold. ![]() The data are derived from the FlyBase GBrowse website ( name=FBgn0037617). (A) The genomic structure of séance and surrounding genes. Generation of séance (CG8145) mutant alleles by the CRISPR/Cas9 system. These results suggest that the three transcription factors have distinct roles in coordinating the expression of just two genes in Drosophila Given that neverland and spookier are located in constitutive heterochromatin, Séan, Ouib, and Mld represent the first example of a transcription factor subset that regulates genes located in constitutive heterochromatin.ĭrosophila ecdysone heterochromatin steroid hormone biosynthesis transcription zinc finger.Ĭopyright © 2018 by the Genetics Society of America. Remarkably, loss-of-function mutations in séan, ouib, or mld can be rescued by the expression of neverland, spookier, or both, respectively. In this study, we report that three zinc finger-associated domain (ZAD)-C 2H 2 zinc finger transcription factors-Séance (Séan), Ouija board (Ouib), and Molting defective (Mld)-cooperatively control ecdysone biosynthesis in the fruit fly Drosophila melanogaster Séan and Ouib act in cooperation with Mld to positively regulate the transcription of neverland and spookier, respectively, two Halloween genes. The expression of the Halloween genes is highly restricted and dynamic, indicating that their spatiotemporal regulation is mediated by their tight transcriptional control. During larval development, ecdysone is synthesized in an endocrine organ called the prothoracic gland through a series of ecdysteroidogenic enzymes encoded by the Halloween genes. 6 Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada 7 Faculty of Life and Environmental Sciences, University of Tsukuba, 305-8572, Ibaraki, Japan 8 Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan.Įcdysteroids are steroid hormones that control many aspects of development and physiology.5 Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia V5A 1S6, Canada.4 Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan.3 Graduate School of Life and Environmental Sciences, University of Tsukuba, 305-8572, Ibaraki, Japan. ![]() 2 Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |